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FC55888

N-Cyclohexyl-N'-(2-morpholinoethyl)carbodiimide methyl-p-toluenesulfonate

N-Cyclohexyl-N'-(2-morpholinoethyl)carbodiimide methyl-p-toluenesulfonate, also known as CMC, CMCT, Morpho-CDI and CME-carbodiimide, is a reagent widely used in the detection of post-transcriptional modifications on nucleosides in RNA. RNA chemical probing is a powerful technology used to address numerous biological questions. The main application of CMC is the chemical modification of pseudouridine in RNA, in order to detect it and quantify it using analytical techniques such as primer extension or MALDI. Pseudouridine is the most abundant modification occurring in cellular RNA, and is responsible for the increased rigidity of the phosphate backbone and base stacking, impacting important RNA interactions.

Technical Data

CAS No: 2491-17-0
Synonyms: Morpho-CDI; CME-carbodiimide
Product Code: FC55888
MDL No: MFCD00011979
Chemical Formula: C21H33N3O4S
Molecular Weight: 423.57

References


  • Carlson et al (2018). SnapShot: RNA Structure Probing Technologies. Cell 157:600-600e1.
  • Zhou et al (2018). Pseudouridines Have Context-Dependent Mutation and Stop Rates in High-Throughput Sequencing. RNA Biology 15:892-900.
  • Patteson et al (2001). Identification of the Mass-Silent Post-Transcriptionally Modified Nucleoside Pseudouridine in RNA by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry. Nucleic Acid Research 29:1-7.

Solubility

Stability

Further Information


Datasheets

Specification: View
MSDS: View

CAS No:
2491-17-0
Synonyms:
Morpho-CDI
CME-carbodiimide
MDL No:
MFCD00011979
Chemical Formula:
Molecular Weight:
423.57
Product Structure
References:
1. Carlson et al (2018). SnapShot: RNA Structure Probing Technologies. Cell 157:600-600e1.
2. Zhou et al (2018). Pseudouridines Have Context-Dependent Mutation and Stop Rates in High-Throughput Sequencing. RNA Biology 15:892-900.
3. Patteson et al (2001). Identification of the Mass-Silent Post-Transcriptionally Modified Nucleoside Pseudouridine in RNA by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry. Nucleic Acid Research 29:1-7.
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