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EB06680

X-Gal

Chromogenic substrate for β-galactosidase generating a blue compound after galactosidase hydrolysis. X-Gal is the substrate of choice in cloning and genetic engineering for selection of recombinant bacterial colonies with the lac+ genotype. It has application in immunohistochemistry.

Technical Data

CAS No: 7240-90-6
Synonyms: 5-Bromo-4-chloro-3-indolyl b-D-galactopyranoside (X-Gal); X-b-D-Galactoside; BCIG
Product Code: EB06680
MDL No: MFCD00005666
Chemical Formula: C14H15BrClNO6
Molecular Weight: 408.63

Literature


  • Arakawa H et al (1991). Chemiluminescent assay of various enzymes using indoxyl derivatives as substrate and its applications to enzyme immunoassay and DNA probe assay. Anal Biochem 199(2):238-42., Weiss DJ et al (1997). In situ histochemical detection of beta-galactosidase activity in lung: assessment of X-Gal reagent in distinguishing lacZ gene expression and endogenous beta-galactosidase activity. Human Gene Therapy 8(13):1545-1554.
  • Engler KH et al (1999). A novel colorimetric yeast bioassay for detecting trichothecene mycotoxins. J Microbiol Methods 35(3):207-18.
  • Joung JK et al (2000). A bacterial two-hybrid selection system for studying protein-DNA and protein-protein interactions. Proc Natl Acad Sci U S A 97(13):7382-7.
  • Sambrook J and Russell DW (2001). Molecular Cloning: A Laboratory Manual, 3rd Edition, Cold Spring Harbor Laboratory Press.
  • Seymour PA and Sander M (2007). Immunohistochemical detection of beta-galactosidase or green fluorescent protein on tissue sections. Methods Mol Biol 411:13-23.

Solubility

Stability

Further Information


Datasheets

Specification: View
MSDS: View
Technical Bulletin: View

X-Gal

CAS No:
7240-90-6
Synonyms:
5-Bromo-4-chloro-3-indolyl b-D-galactopyranoside (X-Gal)
X-b-D-Galactoside
BCIG
MDL No:
MFCD00005666
Chemical Formula:
Molecular Weight:
408.63
Product Structure
References:
1. Arakawa H et al (1991). Chemiluminescent assay of various enzymes using indoxyl derivatives as substrate and its applications to enzyme immunoassay and DNA probe assay. Anal Biochem 199(2):238-42., Weiss DJ et al (1997). In situ histochemical detection of beta-galactosidase activity in lung: assessment of X-Gal reagent in distinguishing lacZ gene expression and endogenous beta-galactosidase activity. Human Gene Therapy 8(13):1545-1554.
2. Engler KH et al (1999). A novel colorimetric yeast bioassay for detecting trichothecene mycotoxins. J Microbiol Methods 35(3):207-18.
3. Joung JK et al (2000). A bacterial two-hybrid selection system for studying protein-DNA and protein-protein interactions. Proc Natl Acad Sci U S A 97(13):7382-7.

4. Sambrook J and Russell DW (2001). Molecular Cloning: A Laboratory Manual, 3rd
Edition, Cold Spring Harbor Laboratory Press.
5. Seymour PA and Sander M (2007). Immunohistochemical detection of beta-galactosidase or green fluorescent protein on tissue sections. Methods Mol Biol 411:13-23.
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